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Registro Completo |
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Biblioteca(s): |
Embrapa Unidades Centrais. |
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Data corrente: |
06/11/2003 |
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Data da última atualização: |
26/10/2018 |
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Autoria: |
TORRES, A. C.; NASCIMENTO, W. M.; PAIVA, S. A. V.; ARAGÃO, F. A. S. de. |
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Afiliação: |
Antonio Carlos Torres, CNPH; Warley Marco Nascimento, CNPH; Sonia Alessandra Vasconcelos Paiva, CNPH; Fernando Antonio Souza de Aragão, CNPH. |
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Título: |
Bioassay for detection of transgenic soybean seeds tolerant to glyphosate. |
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Ano de publicação: |
2003 |
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Fonte/Imprenta: |
Pesquisa Agropecuária Brasileira, Brasília, DF, v. 38, n. 9, p. 1053-1057, set. 2003 |
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Idioma: |
Inglês |
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Notas: |
Título em português: Bioensaio para detecção de sementes de soja transgênicas tolerantes ao glifosato. |
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Conteúdo: |
Glyphosate is a systemic, nonselective, postemergence herbicide that inhibits growth of both weeds and crop plants. Once inside the plant, glyphosate interferes with biosynthesis of aromatic amino acids phenylalanine, tyrosine, and tryptophan, by inhibiting the activity of 5-enolpyruvylshikimate-3-phosphate synthase (EPSPS), a key enzyme of the shikimate pathway. The objective of this work was to develop a simple, effective and inexpensible method for identification of transgenic soybean tolerant to glyphosate. This technique consisted in germinating soybean seeds in filter paper moistened with 100 to 200 mM of glyphosate. Transgenic soybean seeds tolerant to glyphosate germinated normally in this solution and, between 7 and 10 days, started to develop a primary root system. However non-transgenic seeds stopped primary root growth and emission of secondary roots. |
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Palavras-Chave: |
plantas transgênicas; resistência a herbicida. |
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Thesagro: |
Biossíntese; Glycine Max. |
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Thesaurus Nal: |
biosynthesis; herbicide resistance; transgenic plants. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/AI-SEDE/25114/1/v38n9a05.pdf
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Marc: |
LEADER 01722naa a2200253 a 4500
001 1109203
005 2018-10-26
008 2003 bl uuuu u00u1 u #d
100 1 $aTORRES, A. C.
245 $aBioassay for detection of transgenic soybean seeds tolerant to glyphosate.
260 $c2003
500 $aTítulo em português: Bioensaio para detecção de sementes de soja transgênicas tolerantes ao glifosato.
520 $aGlyphosate is a systemic, nonselective, postemergence herbicide that inhibits growth of both weeds and crop plants. Once inside the plant, glyphosate interferes with biosynthesis of aromatic amino acids phenylalanine, tyrosine, and tryptophan, by inhibiting the activity of 5-enolpyruvylshikimate-3-phosphate synthase (EPSPS), a key enzyme of the shikimate pathway. The objective of this work was to develop a simple, effective and inexpensible method for identification of transgenic soybean tolerant to glyphosate. This technique consisted in germinating soybean seeds in filter paper moistened with 100 to 200 mM of glyphosate. Transgenic soybean seeds tolerant to glyphosate germinated normally in this solution and, between 7 and 10 days, started to develop a primary root system. However non-transgenic seeds stopped primary root growth and emission of secondary roots.
650 $abiosynthesis
650 $aherbicide resistance
650 $atransgenic plants
650 $aBiossíntese
650 $aGlycine Max
653 $aplantas transgênicas
653 $aresistência a herbicida
700 1 $aNASCIMENTO, W. M.
700 1 $aPAIVA, S. A. V.
700 1 $aARAGÃO, F. A. S. de
773 $tPesquisa Agropecuária Brasileira, Brasília, DF$gv. 38, n. 9, p. 1053-1057, set. 2003
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Registro original: |
Embrapa Unidades Centrais (AI-SEDE) |
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| 1. |  | TIGANO, M. S.; JEYAPRAKASH, A.; BRITO, J.; CARNEIRO, R. M. D. G.; DICKSON, D. W. Characterization of Meloidogyne floridensis using mitochondrial DNA sequence. In: ANNUAL MEETING of ONTA, 37., 2005, Viña del Mar, Chile. Program and abstracts... [Santiago: Sociedad Chilena de Nematologia], 2005. p. 80. SISPAT 2005 - Produção Técnico-Científica - Resumo em anais de Congresso.| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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